منابع مشابه
Glycoside Hydrolases across Environmental Microbial Communities
Across many environments microbial glycoside hydrolases support the enzymatic processing of carbohydrates, a critical function in many ecosystems. Little is known about how the microbial composition of a community and the potential for carbohydrate processing relate to each other. Here, using 1,934 metagenomic datasets, we linked changes in community composition to variation of potential for ca...
متن کاملREVIEW Glycoside Hydrolases: Catalytic Base/Nucleophile Diversity
Recent studies have shown that a number of glycoside hydrolase families do not follow the classical catalytic mechanisms, as they lack a typical catalytic base/ nucleophile. A variety of mechanisms are used to replace this function, including substrate-assisted catalysis, a network of several residues, and the use of non-carboxylate residues or exogenous nucleophiles. Removal of the catalytic b...
متن کاملBioprospecting metagenomics of decaying wood: mining for new glycoside hydrolases
BACKGROUND To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenomes of natural microbial biomass decay communities may harbor such enzymes. Our goal was to discover and characterize new glycoside hydrolases (GHases) from microbial biomass dec...
متن کاملUse of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases
Chemically synthesized nanostructure-initiator mass spectrometry (NIMS) probes derivatized with tetrasaccharides were used to study the reactivity of representative Clostridium thermocellum β-glucosidase, endoglucanases, and cellobiohydrolase. Diagnostic patterns for reactions of these different classes of enzymes were observed. Results show sequential removal of glucose by the β-glucosidase an...
متن کاملGlycoside hydrolases and glycosyltransferases. Families, modules, and implications for genomics.
One of the first insights into the modular nature of carbohydrate-active enzymes was provided by the dissection of a plant cell wall-degrading enzyme into two functional modules (van Tilbeurgh et al., 1986). The general architecture deduced for this protein featured two independent globular modules: a cellulase catalytic domain, responsible for the hydrolysis reaction itself, and a cellulose-bi...
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ژورنال
عنوان ژورنال: Journal of Molecular Catalysis B: Enzymatic
سال: 2014
ISSN: 1381-1177
DOI: 10.1016/j.molcatb.2014.06.009